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Instrumentation

Cell Analysis Core Facility

BD Pathway

BD PathwayPlatform for High through-put microscopy. Can perform in either confocal or non-confocal mode. Is capable of live cells assays such as calcium flux, live/dead assay, etc with incubator and fluidic functionality. Uses special 96 well/384 well plates or slide format with 4 color capability.

Please click here for additional information about the BD Pathway and Protocol Information!

 

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BD LSRII Analyzer

BD LSRII AnalyzerA multiple laser analyzer. The CACF currently supports 9 parameters, but the LSRII is upgradeable for up to 15 parameters analysis.The standard optical filters on the LSR allow the detection of (but are not limited to) the following fluorophores: PE-Cy7, PE-Cy5.5, PE-TexasRed, PerCP, PerCP-Cy5.5 PE, FITC, PI, APC-Cy7, APC, Hoechst, DAPI, Cascade Blue, Pacific Blue, Alexa Fluor 405 and Alexa Fluor 430. It can simultaneously detect 11 parameters (2 scatter and 9 fluorochromes).

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BD FACSAria

BD FACSAriaA high speed sorter with high purity and yield. The CACF currently supports 9 fluorescent parameters, but the Aria is upgradeable for up to 12 parameters analysis. Cells can be collected under non-aseptic or aseptic conditions into tubes or multi-well plates.

 

 

 

 

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