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Medicine - Pulmonary Diseases, Critical Care & Environmental Medicine - Eosinophilic Disorder Center

Tulane Center for Eosinophilia Disorders under the leadership of Dr. Anil Mishra, PhD, is a first center in the southern region of USA. The center will provide an opportunity to the researchers, patients and their families to understand the disease and consult for the better treatment of the eosinophil associated disorders. The center will also provide an opportunity to the basic research scientist to directly translate their experimental findings to the human disease with the help of clinicians.  The center will be involved in basic, clinical and translational studies to determine the mechanistic aspects of eosinophilic disorders in the lung, skin and multiple gastrointestinal segments. Read More

The center's aim is to develop a diagnostic and therapeutic interventions for eosinophil associated multiple diseases. Eosinophilic disorders occur when eosinophils, white blood cells with a bilobed nucleus with multiple toxic granules white blood cell, are found in above-normal amounts in various parts of the body. At healthy state the eosinophil reside in the gastrointestinal tract; however, during allergic (food or aeroallergen) responses, a large number of eosinophils are generated from their precursors in response to the stimulus and move into the sites (responsive organ of the body) and release a variety of toxins that can cause chronic inflammation, resulting in tissue damage and repair.

The Center and other investigators found that eosinophils have a major role in promoting asthma, dermatitis and a number of gastrointestinal symptoms and tissue remodeling (fibrosis). Because of the growing knowledge and a better diagnostic technology, we now have a better understanding of a number of allergic diseases. We are currently studying multiple compounds that might block eosinophil production and their recruitment into the tissues. Our research work indicated that in the future we could provide the best treatment for eosinophil-associated multiple medical disorders such as asthma, eosinophilic dermatitis, eosinophil associated gastrointestinal disorders and tissue remodeling (fibrosis).

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Basic and Clinical Studies at Our Center

Dr. Mishra's general research is aimed at deciphering mechanisms of inflammation, primarily based on discoveries concerning innate immunity. In particular, gene-environment interactions in the elicitation of inflammatory states in the respiratory and gastrointestinal tracts are under investigation.Read More

Environmental triggers (such as aeroallergens and food allergens) are studied in the context of specific genetic variants (e.g. IL-15 and IL-18 signaling) using population studies (cross sectional and longitudinal prospective cohorts) and mechanism-driven studies.  The biological properties of innate inflammatory cells (eosinophil, mast cells, NKT cells, epithelial cells) and the cytokines (especially chemokines) that mediate their function are under investigation. The contribution of these basic processes to diverse human inflammatory conditions (e.g. asthma, eosinophil associated gastrointestinal disorders) is pursued by translational studies often involving unique clinical resources (e.g. patient samples and databanks) and clinical intervention studies (e.g. anti-cytokine therapeutics). ). We showed an important protective role of IL-15 in allergen or cytokine induced airway obstruction and bronchial fibrosis (JACI 2017 and AJRCMB 2019)

Most Importantly, we first time implicated the role of IL-18 in eosinophils biology including maturing the naïve eosinophils into the CD101/CD274 double positive pathogenic eosinophils (JACI 2018). We also shown a critical role of eosinophils in promoting chronic pancreatitis and first time report a chemical-induced mouse model of pancreatic cancer.

Principal Investigator

Anil Mishra, PhD
Endowed Schlieder Chair and Professor of Medicine, Pulmonary Diseases
Director, Tulane Eosinophilic Disorder Center, Tulane Medical Center, New Orleans, LA 70112
phone: 504-988-3840
fax: 504-988-2144
email: amishra@tulane.edu

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Research Focus Areas

Eosinophilic gastrointestinal Disorders:

Eosinophilic esophagitis, characterized by elevated levels of eosinophil in  the esophagus.

Eosinophilic gastritis, characterized by elevated eosinophil in the stomach.

Eosinophilic enteritis, characterized by elevated eosinophil in the intestine.

Eosinophilic colitis, characterized by excessive eosinophil in the colon.

Eosinophilic Pancreatitis, accumulation of eosinophils in the pancreas

Clinical Collaborators:

Cregory Gaspard, MD (Gastroentrology and Hepatology, Tulane Medical Center, New Orleans, LA)

Mansour A. Parsi, MD (Gastroentrology and Hepatology Section, Tulane Medical center, New Orleans, LA)

 

Asthma, is a chronic inflammatory disease of the airways caused by a combination of genetic and environmental factors and characterized by airflow obstruction, and bronchospasm. Common symptoms include wheezing, coughing, chest tightness, and shortness of breath.

Clinical Collaborators:

Dr. Nereida Parada, MD (Associate Professor, Pulmonary Medicine. Tulane Medical Center, New Orleans, LA)

 

Fibrosis is the formation of excess fibrous connective tissue in an organ or tissue in a reparative or reactive process. This can be a reactive, benign, or pathological state.

Clinical Collaborators:

Dr. Joseph Lasky, MD (Professor and Director, Pulmonary Medicine. Tulane Medical Center, New Orleans, LA)

Fibrosis can occur in many tissues within the body, typically as a result of inflammation or damage, and examples include:

  • Pulmonary fibrosis (lungs) includes, Idiopathic pulmonary fibrosis.
  • Cystic fibrosis.
  • Cirrhosis (liver)
  • Nephrogenic systemic fibrosis (skin)
  • Crohn's Disease (intestine)

Jane M. El-Dhar, MD (Professor of Clinical Pediatrics and Clinical Professor of Medicine, Head, Section of Pediatric Allergy/Immunology/Rheumatology

Dr. Laurianne Wild, MD (Professor and Director, Clinical Immunology, Tulane Medical Center, New Orleans, LA

Dr. John Affornti, MD, Professor of Medicine Interim Chief Division of Gastroenterology & Hepatology

Off Site Collaborative Partner and Consultants

Paul E. Hyman, MD, Professor Pediatrics, Division Head, Children's Hospital, New Orleans, LA 70118. Email: phyman@lsuhsc.edu, Ph 504-896-9334, FAX 504-894-5567

Sandeep K. Gupta, MD, Professor of Clinical Pediatrics & Clinical Medicine Director, Pediatric Over Weight Education and Research Program, Riley Hospital for Children, Indianapolis, IN. email: guptas@iupui.edu

Manoj R. Warrier, MD, Allergy, Asthma & Sinus Care Center 12818 Tesson Ferry Road, Suite #103, St. Louis, MO 63128; Phone:1-314-849-8700, ;  Fax: 314.849.8737; email. m_warrier@hotmail.com

Hamid A. Boulares, PhD, Professor of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center; New Orleans, LA 70112.Phone: 504-210-2233    Email: hboulr@lsuhsc.edu     

 

Future clinical trials

  1. rIL-15 therapy for lung and esophageal fibrosis.
  2. rIL-15 therapy for airway restrictions (airway hyperactivity).
  3. Anti-CD1d and anti-Vα24Jα18 therapy to target iNKT cells for the treatment of eosinophilic esophagitis.
    Tacrolimus trial on eosinophil associated diseases

Patients and Family

Patients and families are encouraged to contact our center for the information needed to understand disease pathogenesis or treatment procedure for eosinophil related disorders.

Contact

Tulane Eosinophilic Disorder Center
Phone: 504-988-3840
Fax: 504-988-2144

Principal Investigator:  Anil Mishra, PhD
Email: amishra@tulane.edu

Clinical Coordinator:  Christine Glynn, RN
Email: cglynn1@tulane.edu
phone:504-988-0743

Gift/Donation

Funds needed for diagnostic and therapeutic Interventions.

Currently, we have very limited support from NIH and our research is very limited. We are in a need of funds to support of research program to find a permanent cure for eosinophil related lung skin and gastrointestinal disorders. We appeal to the common people and private funding agencies to provide the donations for our research programs.

Our Research Highlights

  1. Eosinophils are the resident cell that home prenatally in gastrointestinal tract. J. Clin. Invest. 1999
  2. Developed a murine model of asthma associated eosinophilic esophagitis. J. Clin. Invest. 2001
  3. Esophageal remodeling develops as a consequence of tissue specific IL-5 induced eosinophilia. Gastroenterology. 2008
  4. Interleukin-15 Expression Is Increased in Human eosinophilic esophagitis and Mediates Pathogenesis in Mice. Gastroenterology. 2010
  5. Esophageal functional impairments in experimental eosinophilic esophagitis. AJP-Gastroenterology & Liver Physiol 2009
  6. Invariant natural killer T cells neutralization is a possible novel therapy for human eosinophilic esophagitis. Clinical and Translational Immunology, 2014
  7. Significance of mouse models in dissecting the mechanism of human eosinophilic gastrointestinal diseases (EGID). J Gastroenterology and Hepatology 2013
  8. Resistin-like molecule (Relm)-a induction in eosinophilic esophagitis promotes esophageal epithelial cell hyperplasia. AJP-Gastroentrology. 2014
  9. Involvement of Interleukin-18 in the pathogenesis of human eosinophilic esophagitis. Clinical Immunology, 2015
  10. Possible Noninvasive Biomarker of Eosinophilic Esophagitis: Clinical and Experimental Evidence. Case Reports in Gastroenterology. 2016
  11. Regulatory effects of Interleukin (IL)-15 on allergen-induced airway obstruction. J. Allergy and Clinical Immunology 2017. pii: S0091-6749(17)30926-0. DOI: 10.1016/j.jaci.2017.05.025
  12. Manohar M, Verma AK, Ventateshaiah SU, Mishra A. Role of eosinophils in the initiation and progression of pancreatitis pathogenesis. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2018; 314(2): G211-G222. DOI: 10.1152/ajpgi.00210.2017
  13. Venkateshaiah SU, Mishra, Akanksha, Manohar M, Verma AK, Rajavelu P, Niranjan R, Wild LG, Parada NA, Blecker B, Lasky JA and Mishra A. Role of IL-18 in differentiation, maturation, activation and transformation of pathogenic eosinophils. J Allergy. Clin. Immunol 2018, 142-301-305
Past and Present Laboratory Members

  1. James Schlotman, MD. Joined in 2004 after completing his undergraduate (BS) from Drexel University, Philadelphia. James joined my laboratory at Cincinnati Children’s Hospital Medical Center, Cincinnati and worked more than two years on T cells role in EoE and Resistin like molecule-beta on remodeling till June 2007. He left the laboratory after getting admission in University of Cincinnati College of Medicine, Cincinnati, Ohio and completed MD in 2011-12. He is currently working as a physician at College of Medicine in Houston, Texas.
  2. Zeenath Unnisa, PhD. Joined in 2004 after completing her PhD from Osmania University, Hyderabad, India. Zeenath, worked in my laboratory at Cincinnati Children’s Hospital Medical Center, Cincinnati as a postdoctoral fellow and worked on mast cell role in EoE. Current position and place of work not known.
  3. Venkatarajani R, MS. Joined as a research assistant in my laboratory at Cincinnati Children’s Hospital Medical Center, Cincinnati and worked on esophageal remodeling. Current, position and place of work is not known.
  4. Mequin Wang, MD, PhD. Joined in 2005 as a research associate after completing her PhD from John Hopkins School of Medicine, Baltimore. Mequin, worked in my laboratory at Cincinnati Children’s Hospital Medical Center, Cincinnati till 2008 on esophageal remodeling in EoE and regulatory T cells role in EoE. Currently she is working as Assistantant professor at Children’s Hospital, Denver, CO.
  5. Calab H. Crump, BS. Joined in 2005 as a research assistant from University of Cincinnati after completing his undergraduate studies. Calab, worked in my laboratory at Cincinnati Children’s Hospital Medical Center, Cincinnati till 2008. He re-joined law college and currently doing private practice.
  6. Xiang Zhu,PhD. Joined in 2007 as a postdoctoral fellow, in the University of Cincinnati. Xiang, moved to my laboratory at Cincinnati Children’s Hospital Medical Center, Cincinnati in 2009 and worked on the role of IL-15 and regulatory T cells in EoE. Dr. Zhu is currently working as junior faculty Cincinnati Children’s Hospital, Cincinnati, OH.
  7. Parm Mavi, MS. Joined in 2010 as a research assistant, from University of Cincinnati after completing BS. Parm, worked in my laboratory at Cincinnati Children’s Hospital Medical Center, Cincinnati till 2012 on mast cells and eosinophil role in the development of esophageal functional abnormalities in EoE. Parm recently completed MD and working as resident at University of Cincinnati College of Medicine, Cincinnati, OH.
  8. Akhilesh Pandey, PhD. Joined in 2012 as a visiting postdoctoral fellow for 3 months in my laboratory at Cincinnati Children’s Hospital Medical Center, Cincinnati and currently working as an Instructor at Garner’s Institute of Aging in Texas.
  9. Shankar Narayann, BS. Joined in 2011 as a research assistant after graduating from Ohio State University. Shankar, worked in my laboratory at Cincinnati Children’s Hospital Medical Center, Cincinnati till 2011 and currently completing his Medical Degree (MD) at Wright College of Medicine, Dayton, Ohio.
  10. Priya Rajuvelu, PhD. Joined in 2009 as a research associate, from University of Cincinnati after 6 months of postdoctoral training in Dr. Chaubey’s laboratory. Priya worked in my laboratory at Cincinnati Children’s Hospital Medical Center, Cincinnati till 2012 on the role of IL-15 and IL-18 on eosinophil biology and EoE pathogenesis. She is currently working as Assistant Scientist at University of Cincinnati, Ohio.
  11. Madhavi Rayapudi, MBBS, MD. Joined in 2008 as a research associate. She was a Medical graduate from Guntur Medical School, Guntur, AP, India. Madhavi, worked in my laboratory at Cincinnati Children’s Hospital Medical Center, Cincinnati till 2013 on a number of projects that included food and indoor allergen-induced EoE, INKT cells role in EoE and anoninvasive biomarkers for EoE. She is currently doing her fellowship in pathology at Mayo clinic.
  12. Scott Dynda, MS, Joined in 2012 as a research assistant after completing his master degree from Kent State University Kent, OH. Scott worked in my laboratory at Case Western Reserve University, Cleveland till 2013, on the role of IL-15 in lung and intestinal diseases. He is currently working as Laboratory Manager at Cleveland Clinic, Cleveland, Ohio.
  13. Rituraj Niranjan, PhD. Joined in 2011 as a postdoctoral fellow after doing his initial research training at Central Drug Research Center, Lucknow, India and obtained his PhD from Jawaharlal Nehru University, New Delhi, India. Rituraj worked in my laboratory at Cincinnati Children’s Hospital Medical Center and further continued his research to Case Western Reserve University. He worked on the IL-15 biology and its role in allergen-induced airway hyperreactivity and lung fibrosis. Currently, he is working as Scientist-C at Indian Institute of Medical Research, India.
  14. Parmesh Dutt, PhD. Joined in 2012 as a postdoctoral fellow after losing a grant and closing of FDA sponsored research laboratory at Washington DC. He worked in my laboratory at case Western and Tulane University on eosinophilia subsets identification and eosinophilic inflammation in lung and esophagus. Currently joined Food and Drug Administration, USA as a Scientist.
  15. Asifa Zaidi, PhD, Joined in 2012 as a postdoctoral fellow after losing a postdoctoral job at John Hopkins School of Medicine due to loss of NIH funding by his supervisor. Asifa obtained her PhD in biosciences from Bhabha Atomic Research Center, Mumbai, India and had her postdoctoral training at John Hopkins Medical School, Baltimore. She left in 2014 and currently working as Statistical analysis at Case Western reserve University, Cleveland, OH.
  16. Aditi Mathur, PhD Aditi joined our laboratory as a postdoctoral fellow in 2014 and moved to Chicago, IL due to the some family reason. Aditi obtained his PhD from Tulane University School of Medicine, New Orleans, LA in 2014 and recently joined Eosinophilic Disorder Center at New Orleans, LA. Current place and position is not known.
  17. JaiShanker Shukla, PhD Jaishankar obtained his PhD from Kanpur University, India and he is a staff member at Sanjay Gandhi Postgraduate Medical Institute at Lucknow, India, India. He came to my laboratory for a year on study leave to learn some molecular and immunology techniques in December 2013 and worked till 2015. He is currently working at Staff Member at Sanjay Gandhi Post Graduate Institute (SGPGI) Lucknow, India.
  18. Rakesh Mishra, PhD., Postdoctoral fellow. Joined in 2014 as a postdoctoral fellow to work on the role of IL-18 and IL-15 celiac disease. Rakesh obtained his PhD from Patel Chest Research Center, New Delhi, India and completed two years of postdoctoral training at University of Illinois, Chicago. Currently placed in New York, NY
  19. Ruijuan Gao, PhD. Postdoctoral fellow. She joined our laboratory in 2014 as a Research Associate and currently working as Data Analyst in a private farm at Florida.
  20. Siddesha J. Mariswamy, PhD., Postdoctoral fellow. Siddesha obtained his PhD from University of Mysore, Mysore, Karnataka, India, India and has done his postdoctoral training at NIH and joined Tulane eosinophilic disorder center in 2014.
  21. Anshi Shukla, PhD., Postdoctoral fellow. Anshi completed her PhD degree from BHU, Varanasi, India. Joined our laboratory in 2014 as a pre-doctoral fellow and currently moved to the Transplant Clinic, Washington University, Washington, DC.
  22. Dewan Majid, MD, Joined recently Tulane eosinophilic Disorder Center after completing MD degree from Tulane University School of Medicine, New Orleans, LA. Dr. Majid is doing Residency at St. Louis, MS
  23. Chandrashekara P. Madhadevappa, PhD Joined Tulane Eosinophilic disorder Center in July 2015 and worked on CXCR4/SDF1 pathway in EMT associated esophageal functional disorders in EoE. Chandrashekhera obtained his PhD degree from University of Mysore, Mysore, Karnataka, India, India. Dr. Madhadevappa moved back to India in July 2016 joined faculty position in Mysore, India maturation. Sathisha, obtained his PhD degree from University of Mysore, Mysore, Karnataka, India, India. Sathisha, moved to University of Missouri, Columbia, MO
  24. Murli Manohar, PhD. Joined Tulane Eosinophilic disorder center in May 2015 and worked on the role of IL-15 in the pathogenesis of acute and chronic pancreatic fibrosis. Murli, obtained his PhD from Central drug Research institute (CSIR) Lucknow, India. Currently Dr Manohar moved in Feb 2018 to Standford University, San Francisco, CA for his further training in research.
  25. Alok K. Verma, PhD, Joined Tulane Eosinophilic Disorder Center in July 2015 and working on the role of vasoactive intestinal peptide in eosinophil trafiking in the muscular mucosa. Dr. Verma graduated in 2014 and obtained his PhD degree from Indian Toxicology research Center, Lucknow, India. Dr. Verma moved to Cincinnati Children’s Hospital Medical Center in 2018.
Students Past/Present

The following high school/graduate students were introduced to medical research in summer (June-August) of all years and trained in my laboratory.

  1. Kalyan M. Rao, 2008 May-July, Currently working as Statistical Analyst at Proctor and Gamble, Cincinnati, Ohio
  2. Srichandan Rajmouli, 2008 May-July, Currently Medical student at Collage of Medicine University of Cincinnati, OH
  3. Mathew Moffitt, 2009 May-July, Valpariso University, Indiana. Currently Medical student in Indiana.
  4. Birva Dave, 2009 May-July, Anderson High School, Cincinnati, Ohio. Currently working as Pharmacist in Florida
  5. Anupreet K.Lekhi, 2010 May-July, Ohio State University, Columbus, Ohio.
  6. Archit Sahay, 2012 May-July, Ohio State University, Columbus, Ohio. Currently doing medical education in Toledo, Ohio
  7. Akanksha Mishra, 2012 June-August, University of Cincinnati, Ohio. Currently doing her medical studies.
  8. Prithvi Jami, 2013 JUNE-AUGUST, Currently doing medical education in Toledo, Ohio
  9. Ahaad Mushad, 2014,November-June 2014. Independent research student , currently doing his medical education in LSU.
  10. Nathan Sanders 2015-16 Independent research student to complete his undergraduate course for Tulane University, New Orleans, LA Currently doing his academic medical education (MD, PhD) in Boston, MA
Current Faculty and Postdoctoral Fellow

  1. Sathisha U. Venkateshaiah, PhD. Joined the Tulane eosinophilic disorder center in July 2014 and worked on the IL-15 role in airway resistance and compliance following the induction of experimental asthma. Additionally he showed a critical role of IL-18 in eosinophils. Dr. Venkateshaiah is now promoted to the faculty position at Tulane School of Medicine and he is now managing my laboratory research and guiding new postdoctoral fellows and students.
  2. Hemanth K. Kandikattu, PhD Joined Tulane Eosinophilic Disorder Center in March 2018 and working on pathogenesis of acute and chronic eosinophil associated pancreatic fibrosis and Malignancy. Hemanth obtained his PhD from the Defense Food Research laboratory/University of Mysore, Karnataka, India. Before joining my laboratory Hemath worked at University of Missouri, Columbia, Missouri from 2015 to March 2018 on the project involving the role of RECK (Reversion inducing cysteine rich protein with kazal motifs) and TRAF3IP2 in cardiac hypertrophy, fibrosis and obesity.
  3. Jaya Mishra. PhD. Clinical Coordinator. She has a vast experience of basic and clinical research and currently involved in the translational research work of our laboratory.
Current Student

  1. Arjun Verma, 2016- Independent research student to complete his undergraduate course for Tulane University, New Orleans, LA