How do I check cell viability after tissue digestion?
The core has a Nexcelom Cellometer Auto 200 that can provide cell density and % viability.
How do I handle samples where access to the 10X Chromium Controller may not be immediately available?
10X has developed a protocol for methanol fixation that allows later processing of the sample.
How can I process multiple samples cost effectively?
The Satija lab at NY Genome Center has developed cells hashing that uses bar coded antibodies to pool samples.
Original Papers
Article on Genome Biology in BioMedCentral