How do I check cell viability after tissue digestion?
The core has a Nexcelom Cellometer Auto 200 that can provide cell density and % viability.
How do I handle samples where access to the 10X Chromium Controller may not be immediately available?
10X has developed a protocol for methanol fixation that allows later processing of the sample.
How can I process multiple samples cost effectively?
The Satija lab at NY Genome Center has developed cells hashing that uses bar coded antibodies to pool samples.
Original Papers:
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1603-1
https://cite-seq.com/cell-hashing/
Commercial protocol/products:
https://www.biolegend.com/NewsLegend/091818hashtags/index.htm
