Instrumentation

Cell Analysis Core Facility

AMNIS MarkII Imaging Flow Cytometer

Speed and sample size of flow cytometry with the resolution and sensitivity of microscopy in a single instrument platform unlike any other available for cell analysis. With up to 12 channels (up to 10 colors, 2 brightfield) for each cell in a population, microscopic images provide qualitative and quantitative image data of every event acquired in flow. Has 20x, 40x, 60 objectives. Contact core for details.

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BD LSRII Analyzer

A multiple laser analyzer. The CACF currently supports 9 parameters, but the LSRII is upgradeable for up to 15 parameters analysis.The standard optical filters on the LSR allow the detection of (but are not limited to) the following fluorophores: PE-Cy7, PE-Cy5.5, PE-TexasRed, PerCP, PerCP-Cy5.5 PE, FITC, PI, APC-Cy7, APC, Hoechst, DAPI, Cascade Blue, Pacific Blue, Alexa Fluor 405 and Alexa Fluor 430. It can simultaneously detect 11 parameters (2 scatter and 9 fluorochromes).

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BD FACSAria III

A high speed sorter that is capable of sorting up to four different cell populations into a variety of tubes or a single population directly into multi-well plates under sterile or non-sterile conditions based on the application.  It has 488, 561, 633 and 405 laser lines.  Can run 12 colors. 

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