Single Cell FAQs

The core has a Nexcelom Cellometer Auto 200 that can provide cell density and % viability. 

https://www.nexcelom.com/nexcelom-products/cellometer-fluorescent-viability-cell-counters/cellometer-auto-2000/

10X has developed a protocol for methanol fixation that allows later processing of the sample.

https://support.10xgenomics.com/single-cell-gene-expression/sample-prep/doc/demonstrated-protocol-methanol-fixation-of-cells-for-single-cell-rna-sequencing

The Satija lab at NY Genome Center has developed cells hashing that uses bar coded antibodies to pool samples.

Original Papers:

https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1603-1

https://cite-seq.com/cell-hashing/

Commercial protocol/products:

https://www.biolegend.com/NewsLegend/091818hashtags/index.htm