How do I check cell viability after tissue digestion? The core has a Nexcelom Cellometer Auto 200 that can provide cell density and % viability. https://www.nexcelom.com/nexcelom-products/cellometer-fluorescent-viability-cell-counters/cellometer-auto-2000/ How do I handle samples where access to the 10X Chromium Controller may not be immediately available? 10X has developed a protocol for methanol fixation that allows later processing of the sample. https://support.10xgenomics.com/single-cell-gene-expression/sample-prep/doc/demonstrated-protocol-methanol-fixation-of-cells-for-single-cell-rna-sequencing How can I process multiple samples cost effectively? The Satija lab at NY Genome Center has developed cells hashing that uses bar coded antibodies to pool samples. Original Papers: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1603-1 https://cite-seq.com/cell-hashing/ Commercial protocol/products: https://www.biolegend.com/NewsLegend/091818hashtags/index.htm
