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Bo Ning, PhD
Bo Ning, PhD

Biography

I started my biochemistry and molecular biology studies since my Ph.D. Using myelodysplastic syndromes (MDS) cells, I found a novel DNA methylation mechanism during hematopoietic stem cell (HSC) differentiation. As a postdoctoral fellow in Houston Methodist Research Institute, I expanded my research to histone epigenetic modifications, such as methylation and ubiquitination in induced pluripotent stem cell (iPSC). Also, I am working on how cancer cells deliver their drug resistance or metastasis to other cells through exosomes. I have developed a Quantum-Dot based assay to profile pancreatic cancer biomarkers from single exosome. I also identified a novel exosome marker in Non-Small Cell Lung Cancer (NSCLC) to predict distant metastasis after chemotherapy. Since COVID-19 outbroke in 2020, I keep developing CRISPR based detection technology for COVID-19 clinical diagnosis. Using RT-PCR and RT-RPA CRISPR, our method shows higher sensitivity than qRT-PCR in patient nasal swab, saliva and blood samples. With the full evaluation of clinical performance at Tulane Hospital Molecular Pathology Laboratory, this method has been submitted an Emergence Use Authorization (EUA) for approval. My current research involves body fluid test method development for infectious disease diagnosis including COVID-19.

 

Representative publications

Ning, B., Huang, Z., Youngquist, B.M. Scott J, Niu A, Zwezdaryk K, Bojanowski C, Cao J, Saba N, Yin X, Fan J, Lyon C, Li C, Roy C, Hu Y. Liposome-mediated detection of SARS-CoV-2 RNA-positive extracellular vesicles in plasma. Nature. Nanotechnology. 2021.Corresponding author Link

Huang, Z.,  LaCourse, SM., Kay, AM, Stern J.,  Escudero, JN. Youngquist, BY., Zheng, W. Vambe, D.,  Dlamini, V,  Mtetwa, G. , Cranmer, LM,  Njuguna, I., Wamalwa, DC.,  Maleche-Obimbo, E,  Catanzaro, DG.,  Lyon, CJ, John-Stewart, G,  DiNardo, A.,  Mandalakas,AM, Ning, B., &  Hu, T.Y. CRISPR detection of circulating cell-free Mycobacterium tuberculosis DNA in adults and children, including children with HIV: a molecular diagnostics study. Lancet Microbe, 2022.

Ning, B., Yu, T., Zhang, S., Huang, Z., Tian, D., Lin, Z., Niu, A., Golden, N., Hensley, K., Threeton, B., Lyon, C. J., Yin, X., Saba, N., Rappaport, J., Wei, Q., T.Y.Hu. A smartphone-read ultrasensitive and quantitative saliva test for COVID-19. Science Advances. 2021. 7 (2), eabe3703.

Huang, Z., Ning, B., Youngquist, B., Niu, A., Lyon, C., Beddingfield, B., Fears, A., Monk, C., Murrell, A., Bilton, S., Linhuber, J., Norton, E., Dietrich, M., Lai, W., Scott, J., Yin, X., Rappaport, J., Robinson, J., Saba, N., Roy, C., Zwezdaryk, K., T. Y. Hu, Sensitive tracking of viral RNA in plasma through all stages of SARS-CoV-2 infection. J. Clinical Investigation. 2021.

Huang, Z., Tian, D., Liu, Y., Lin, Z., Lyon, C., J., Lai, W., Fusco, D., Drouin, A., Yin, X., Hu Y*, Ning, B.*. Ultra-Sensitive High Throughput CRISPR Diagnosis of COVID-19. Biosensor and Bioelectronics. 2020, September 164, (15) 112316. Corresponding author.

Liu Y, Fan J, Xu T, Ahmadinejad N, Hess K, Lin S, Zhang J, Liu X, Liu X, Ning B*, Liao Z*, Hu, Y*. Extracellular Vesicle Tetraspanin-8 Expression Predicts Distant Metastasis in Non-Small Cell Lung Cancer after Concurrent Chemoradiation, Science Advances, 2020. 6 (11), eaaz6162 Corresponding author

Publications

Since 2010

  1. Ning, B., Huang, Z., Youngquist, B.M. Scott J, Niu A, Zwezdaryk K, Bojanowski C, Cao J, Saba N, Yin X, Fan J, Lyon C, Li C, Roy C, Hu Y. Liposome-mediated detection of SARS-CoV-2 RNA-positive extracellular vesicles in plasma. Nature. Nanotechnology. 2021.
  2. Ning, B., Yu, T., Zhang, S., Huang, Z., Tian, D., Lin, Z., Niu, A., Golden, N., Hensley, K., Threeton, B., Lyon, C. J., Yin, X., Saba, N., Rappaport, J., Wei, Q., T.Y.Hu. A smartphone-read ultrasensitive and quantitative saliva test for COVID-19. Science Advances. 2020. Accepted.
  3. Huang, Z., Ning, B., Youngquist, B., Niu, A., Lyon, C., Beddingfield, B., Fears, A., Monk, C., Murrell, A., Bilton, S., Linhuber, J., Norton, E., Dietrich, M., Lai, W., Scott, J., Yin, X., Rappaport, J., Robinson, J., Saba, N., Roy, C., Zwezdaryk, K., T. Y. Hu, Sensitive tracking of viral RNA in plasma through all stages of SARS-CoV-2 infection. J. Clinical Investigation. 2021. In press.
  4. Pal P., Ibrahim M, Niu A., Zwezdaryk KJ., Tatje E., Robinson W R., Ko L, Satyavarapu I, W E. J, Nachabe A, Luk A, Mushatt D M., Halvorson K, Denson J L., Smith C C., Simeone F, Davis G, S K. G, McDougal A, Vigh A S., Peterson T G., Ning B., Hu T, F Socola, Robinson Safah J, H and Saba N. S., Safety and efficacy of COVID-19 convalescent plasma in severe pulmonary disease: A report of 17 patients, Transfusion Medicine, 2020 October. In press  
  5. Zhu M, Deng G, Tan P, Xing C, Guan C, Jiang C, Zhang Y, Ning B, Li C, Yin B, Chen K, Zhao Y, Wang YH, Levine B, Nie G, and Wang RF. Beclin 2 negatively regulates innate immune signaling and tumor development, J Clin Invest. 2020 August. In press. 
  6. Huang, Zhen; Tian, Di; Liu, Yang; Lin, Zhen; Lyon, Christopher; Lai, Weihua; Fusco, Dahlene; Drouin, Arnaud; Yin, Xiaoming; Hu, Tony*; Bo Ning*, Ultra-sensitive and high-throughput CRISPR-Powered COVID-19 diagnosis, Biosensors and Bioelectronics, 112316, 2020 (Corresponding author)
  7. Alex Niu, April McDougal, Bo Ning, Firas Safa, Alfred Luk, David M Mushatt, Adeem Nachabe, Kevin J Zwezdaryk, James Robinson, Tim Peterson, Francisco Socola, Hana Safah, Tony Hu, Nakhle S Saba, COVID-19 in allogeneic stem cell transplant: high false-negative probability and role of CRISPR and convalescent plasma, Bone marrow transplantation, 2020
  8. Liu, Yang; Gu, Yajun; Ng, Serina; Deng, Zaian; Lyon, Christopher J; Koay, Eugene J; Ning, Bo; Katz, Matthew H; Chiao, Paul J; Fan, Jia; Circulating levels of hydroxylated bradykinin function as an indicator of tissue HIF-1α expression, Science Bulletin, 2020
  9. Yang Liu, Jia Fan, Ting Xu, Navid Ahmadinejad, Kenneth Hess, Steven H. Lin, Jianjun Zhang, Xi Liu, Li Liu1, Bo Ning*, Zhongxing Liao, Tony Y. Hu, •      Extracellular Vesicle Tetraspanin-8 Level Predicts Distant Metastasis in Non-Small Cell Lung Cancer after Concurrent Chemoradiation, Science Advances, 2019, minor revision (corresponding author)
  10. Deng Z, Zhao Z, Ning Bo, Basilio J, Mann K, Fu J, Gu Y, Ye Y, Wu X, Fan J, Chiao P, Hu TY, Nanotrap-enabled quantification of KRAS-induced peptide hydroxylation in blood for cancer early detection, Nano Research, 12, 6, pp 1445–1452
  11. F Zhang, CJ Lyon, RJ Walls, Bo Ning, J Fan, TY Hu, Cathepsin B Dependent Cleavage Product of Serum Amyloid A1 Identifies Patients with Chemotherapy-Related Cardiotoxicity, ACS Pharmacology & Translational Science. 2019
  12. M Rodrigues, N Richards, Bo Ning, CJ Lyon, TY Hu, A rapid lipid-based approach for normalization of quantum dot-detected biomarker expression on extracellular vesicles in complex biological samples, Nano Letters, 2019,
  13. MH Wan, Bo Ning, S Spiegel, CJ Lyon, TY Hu, Tumor‐derived exosomes (TDEs): How to avoid the sting in the tail, Medicinal research reviews, 2019,
  14. Zhen Zhao, Bo Ning, Christopher.J.Lyon and Tony Hu, Extracellular Vesicles as Cancer Liquid Biopsies: from discovery to clinical application, Lab On a Chip, 2019, 19 (7), 1114-1140
  15. Ji T, Lang J, Ning B (co-first author), Qi F, Wang H, Zhang Y, Zhao R, Yang X, Zhang L, Li W, Shi X, Qin Z, Zhao Y, Nie G. Enhanced Natural Killer Cell Immunotherapy by Rationally Assembling Fc Fragments of Antibodies onto Tumor Membranes. Advanced Materials. 2018 Dec 14;.
  16. Fan J, Wei Q, Koay EJ, Liu Y, Ning B, Bernard PW, Zhang N, Han H, Katz MH, Zhao Z, Hu Y. Chemoresistance Transmission via Exosome-Mediated EphA2 Transfer in Pancreatic Cancer. Theranostics. 2018;8(21):5986-5994.
  17. Zhao W, Zhu Q, Tan P, Ajibade A, Long T, Long W, Li Q, Liu P, Ning B, Wang HY. Tgfbr2 inactivation facilitates cellular plasticity and development of Pten-null prostate cancer. Journal of molecular cell biology. 2018;10(4):316-30.
  18. Long W, Zhao W, Ning B, Huang J, Chu J, Li L, Ma Q, Xing C, Wang HY, Liu Q. PHF20 collaborates with PARP1 to promote stemness and aggressiveness of neuroblastoma cells through activation of SOX2 and OCT4. Journal of molecular cell biology. 2018;10(2):147-60.
  19. Ning B, Zhao W, Qian C, Liu P, Li Q, Li W, Wang R-F. USP26 functions as a negative regulator of cellular reprogramming by stabilising PRC1 complex components. Nature communications. 2017;8(1):349.
  20. Fan J, Ning B, Lyon CJ, Hu TY. Circulating Peptidome and Tumor-Resident Proteolysis.  The Enzymes: Elsevier; 2017. p. 1-25.
  21. Liu F, Huang J, Ning B, Liu Z, Chen S, Zhao W. Drug discovery via human-derived stem cell organoids. Frontiers in pharmacology. 2016;7:334.
  22. Ning B, Li W, Zhao W, Wang R. Targeting epigenetic regulations in cancer. Acta biochimica et biophysica Sinica. 2015;48(1):97-109.
  23. Zhao W, Ning B, Qian C. Regulatory factors of induced pluripotency: current status. Stem cell investigation. 2014;1.
  24. Zhang Z, Zhang F, An P, Guo X, Shen Y, Tao Y, Wu Q, Zhang Y, Yu Y, Ning B. Ferroportin1 deficiency in mouse macrophages impairs iron homeostasis and inflammatory responses. Blood. 2011:blood-2011-01-330324.
  25. Ning B, Liu G, Liu Y, Su X, Anderson GJ, Zheng X, Chang Y, Guo M, Liu Y, Zhao Y. 5-aza-2'-deoxycytidine activates iron uptake and heme biosynthesis by increasing c-Myc nuclear localization and binding to the E-boxes of TfR1 and ferrochelatase genes. Journal of Biological Chemistry. 2011:jbc. M111. 258129.
  26. Fan J, Yin J-J, Ning B, Wu X, Hu Y, Ferrari M, Anderson GJ, Wei J, Zhao Y, Nie G. Direct evidence for catalase and peroxidase activities of ferritin–platinum nanoparticles. Biomaterials. 2011;32(6):1611-8.
  27. Zhang Y, Mikhael M, Xu D, Li Y, Soe-Lin S, Ning B, Li W, Nie G, Zhao Y, Ponka P. Lysosomal proteolysis is the primary degradation pathway for cytosolic ferritin and cytosolic ferritin degradation is necessary for iron exit. Antioxidants & redox signaling. 2010;13(7):999-1009.
  28. Shi Z-H, Nie G, Duan X-L, Rouault T, Wu W-S, Ning B, Zhang N, Chang Y-Z, Zhao B-L. Neuroprotective Mechanism of Mitochondrial Ferritin on 6-Hydroxydopamine–Induced Dopaminergic Cell Damage: Implication for Neuroprotection in Parkinson's Disease. Antioxidants & redox signaling. 2010;13(6):783-96.
  29. Ning B, Liu G, Anderson G, Nie G. TfR1 and Ferrochelatase Expression During Erythroid Differentiation Induced by 5-Aza-Cdr In Both MEL Cells and BFU-E-Derived Erythroblasts Correlates with c-Myc Function. American Society of Hematology; 2010.
  30. Nie G, Zhu M, Ning B. Applications of Nuclear Analytical Techniques for Iron-omics Studies. Nuclear Analytical Techniques for Metallomics and Metalloproteomics, 2010. p. 239-64.