Research

My research is focused on studying the cellular and molecular mechanisms that regulate acid-base balance and intracellular pH particularly those relevant to renal epithelia. These studies involve using basic molecular biology techniques to express transporters and/or channels in frog oocytes and other mammalian cells in order to study the properties of these transport mechanisms. The physiological methods we have been using include ion-selective microelectrodes, two-electrode voltage clamp, optical measurements of intracellular pH by fluorescence and the isolated perfused kidney tubule...