Bilon Khambu, M.Sc., Ph.D.

Assistant Professor

Tulane Cancer Center Member
School of Medicine
Department
Pathology Laboratory Medicine
Tulane Cancer Center

Education & Affiliations

2011-2014 - Postdoctoral Research Fellow, Department of Pathology and Laboratory Medicine, School of Medicine, Indiana University, USA Research Focus: Autophagy, Liver Injury, Inflammation, Fibrosis, and cancer. Mentor: Prof. Xiao-Ming Yin
2008-2011 - PhD in Medicine, Graduate School of Medicine Kyoto University, Japan. Laboratory: Chemical Biology, Mentor: Prof. Motonari Uesugi
2007-2008 - Pre-doctoral Research student, Department of Biochemistry, Kyoto University, Japan
2005 - MSc (Medical Biochemistry), B. P. Koirala Institute of Health Sciences, Nepal
2001 - BSc (Major: Microbiology), Tribhuvan University, Nepal

Biography

Professional Positions and Working Experience

 

2005-2007 - Lecturer, Department of Biochemistry, Kathmandu University Medical School (KUMS), Dhulikhel, Nepal

 

2007-2008 - Predoctoral research student, Chemical Biology laboratory, Department of Biochemistry, School of medicine, Kyoto University. (Mentor: Prof. Motonari Uesugi)

 

2008-2011 - Doctoral student, Chemical Biology Laboratory, Department of Biochemistry, School of medicine, Kyoto University. (Mentor: Prof. Motonari Uesugi)

 

2011-2014 - Postdoctoral fellow, Department of Pathology and Laboratory Medicine, Indiana University School of Medicine. (Mentor: Prof. Xiao-Ming Yin)

 

2014-2015 - Senior Research fellow, Department of Pathology and Laboratory Medicine, Indiana University School of Medicine. (Mentor: Prof. Xiao-Ming Yin)

 

2015-2020 - Research Assistant Professor, Department of Pathology and Laboratory Medicine, Indiana University School of Medicine.

 

2020-Present - Assistant Professor (Tenure-Track), Department of Pathology and Laboratory Medicine, Tulane University School of Medicine.

Research

Original Research

  1. Khambu B*, Hong H, Liu S, Liu G, Chen X, Dong Z, Wan J, and Yin XM.  The HMGB1-RAGE axis modulates the growth of autophagy-deficient hepatic tumors. Cell Death and Disease, 2020, In Press. * Corresponding author
  2. Khambu B, L.T., Yan S, Yu C, Chen X, Goheen M, Li Y, Lin J, Cummings OW, Lee YA, Friedman S, Dong Z, Feng GS, Wu S, Yin XM, Hepatic Autophagy Deficiency Compromises FXR Functionality and Causes Cholestatic Injury. Hepatology. 2019; 69(5):2196-2213.
  3. Zhang H, Yan S, Khambu B, Ma F, Li Y, Chen X, Martina JA, Puertollano R, Li Y, Chalasani N, Yin XM. Dynamic MTORC1-TFEB feedback signaling regulates hepatic autophagy, steatosis and liver injury in long-term nutrient oversupply. Autophagy, 2018; 14(10): 1779-1795.
  4. Khambu B, Huda N, Chen X, Antoine DJ, Li Y, Dai G, Dong Z, Zong WX, Waguri S, Köhler UA, Werner S, Oury TD, Yin XM. HMGB1 promotes ductular reaction and tumorigenesis in autophagy-deficient livers. J Clin Invest. 2018; 128(6):2419-2435.
  5. Lee YM, Noon LA, Akat K, Ybanez M, Lee TF, Berres ML, Fujiwara N, Goossens N, Chou H, Parvin-Nejad F, Khambu B, Kramer E, Gordon R, Pfleger C, Germain D, John G, Campbell K, Yue Z, Yin XM, Cuervo AM, Czaja M, Fiel M, Hoshida Y, and Friedman S. Autophagy is a Gatekeeper of Hepatic Differentiation and Carcinogenesis by Controlling the Degradation of Yap. Nat Commun. 2018; 23(9):4962.
  6. Yu C, Yan S, Khambu B, Chen X, Dong Z, Luo J, Michalopoulos GK, Wu S, Yin XM. Gene Expression Analysis Indicates Divergent Mechanisms in DEN-Induced Carcinogenesis in Wild Type and Bid-Deficient Livers. PLoS One. 2016 May 19;11(5):e0155211.
  7. Li M., Khambu B., Zhang H., Kang JH., Chen X., Chen D., Vollmer L., Liu PQ., Vogt A., and Yin XM. Suppression of lysosome function induces autophagy via a feedback down-regulation of MTOR Complex 1 (MTORC1) activity. Journal of Biological Chemistry, 2013: 288 (50): 35769-35780.
  8. Chen X., Khambu B., Zhang H., Gao W., Li M., Chen X., Yoshimori T., Yin XM. Autophagy induced by calcium phosphate precipitates targets damaged endosomes. Journal of Biological Chemistry, 2013; 289 (16): 11162-11174.
  9. Khambu B., Uesugi M., and Kawazoe Y. Translational repression stabilizes messenger RNA of autophagy-related genes. Genes Cells, 2011; 16(8): 857-6.

Research Support

 

Global center of Excellence (GCOE) research grant, ICR, KU 2008-2011

Title: Chemical biology approach to dissect cell vacuolization process.

Status: Funded

Contributions

Review Articles

  1. Khambu B*., Yan S, Huda N, and Yin X-M.  Role of high-mobility group box-1 in liver pathogenesis.  International Journal of Molecular Sciences, 2019, Int J Mol Sci. 2019 (Accepted) . * Corresponding author
  2. Huda N, Liu G, Hong H, Yan S, Khambu B, Yin XM. Hepatic senescence, the good and the bad. World J Gastroenterol 2019; 25(34): 5069-5081.
  3. Yan S, Khambu B, Hong H, Liu G, Huda N, Yin XM. Autophagy, Metabolism, and Alcohol-Related Liver Disease: Novel Modulators and Functions. Int J Mol Sci. 2019 Oct 11; 20(20). pii: E5029.
  4. Khambu B*, Yan S, Huda N, Liu Gang, Yin XM. Autophagy in non-alcoholic fatty liver disease and alcoholic liver disease. Liver Research, 2018; 2(3):112-119. * Corresponding author
  5. Khambu B, Yan S, Huda N, Gang Liu, Yin XM. Homeostatic Role of Autophagy in Hepatocytes. Seminars in Liver Disease. 2018; 38(04): 308-319.
  6. Asano L, Watanabe M, Ryoden Y, Usuda K, Yamaguchi T, Khambu B, Takashima M, Sato SI, Sakai J, Nagasawa K, Uesugi M. Vitamin D Metabolite, 25-Hydroxyvitamin D, Regulates Lipid Metabolism by Inducing Degradation of SREBP/SCAP. Cell Chem Biol. 2017; 16; 24(2):207-217.
  7. Yan S, Huda N, Khambu B, Yin XM. Relevance of autophagy to fatty liver diseases and potential therapeutic applications. Amino Acids. 2017 Dec;49(12):1965-1979.
  8. Khambu, B, L. Wang, H. Zhang, and X.-M. Yin, The activation and function of autophagy in alcoholic liver disease.  Current Molecular Pharmacology 2017;10(3):165-171.
  9. Klionsky D J,….., Khambu B., et al. Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy, 2016: 12(1): 1-222.
  10. Wang L, Khambu B, Zhang H, Yin XM. Autophagy in alcoholic liver disease, self-eating triggered by drinking. Clin Res Hepatol Gastroenterol. 2015 Sep;39 Suppl 1:S2-6.
  11. Kuo TF., Mao D., Hirata N., Khambu B., Kimura Y.,Kawase E., Shimogawa H., Ojita M., Nakatsuji N, Ueda K., Uesugi M. Selective Elimination of Human Pluripotent Stem Cells by a Marine Natural Product Derivative. Journal of the American Chemical Society, 2014; 136 (28): 9798-9801.

Book Chapters

  1. Khambu B, Zhang H, Yin XM. Role of autophagy and mitophagy in liver disease.  In “Mitochondria in Liver Disease” (ed. By D. Han and N. Kaplowitz, a book of “Oxidative Stress and Disease” series), CRC Press, 2015.
  2. Zhang H, Khambu B, Yin XM. Chapter 12. Autophagy.  In “Signaling Pathways in Liver Diseases” (3rd edition, by J. F. Dufour and P.A. Clavien), Springer, 2015
  3. Khambu B, Huda N, Zou H, Yan S, Yin XM (2017). Autophagy in Liver Homeostasis. In: Ding WX., Yin XM. (eds) Cellular Injury in Liver Diseases. Cell Death in Biology and Diseases. Springer, Cham.
  4. Huda N, Zou H, Yan S, Khambu B, Yin XM (2019) Analysis of Autophagy for Liver Pathogenesis. In: Ktistakis N., Florey O. (eds) Autophagy. Methods in Molecular Biology, vol 1880. Humana Press, New York, NY.

Published Abstracts

  1. Khambu B, Hong H, Liu G, Chen X, Wan J, and Yin XM.  Role of HMGB1 in hepatic tumorigenesis caused by autophagy-deficiency. Hepatology 2019; 70,S1:0270-9139.
  2. Huda N, Khambu B, Chen X, and Yin XM.  Senescence contributes to inflammation in autophagy-deficient livers. Hepatology 2019; 70,S1:0270-9139.
  3. Khambu B, Yan S, Huda N, Liu G, Hong H, Chen X and Yin XM. Autophagy Regulates Bile Acid Metabolism via a NRF2-FXR Signaling Axis. The FASEB Journal 2019; 33(1), 126.3-126.3.
  4. Yan S, Khambu B, Chen X and Yin XM. Autophagy Deficiency in the Liver Altered Pathogenesis of Alcoholic Liver Disease and Profile of Gut Microbiota. The FASEB Journal 2019; 33(1)126.5-126.5.
  5. Khambu B, Huda N, Chen X and Yin XM. A Critical Role of HMGB1 in Liver Pathology Caused By Autophagy Deficiency. Hepatology 2018; 68, 40A-41A.
  6. Huda N, Khambu B, Chen X and Yin XM.  Autophagy Orchestrates Senescence in the Liver. Hepatology 2018; 68, 881A-882A.
  7. Yin XM, Khambu B, Huda N. A critical role of HMGB1 in liver tumor development under autophagy deficiency. Cancer Biology & Medicine 2018; 15(1).4.
  8. Yin XM, Wang L, Yan S, Zhou J, X Chen J, Khambu B. SQSTM1 is important for ethanol-induced lipophagy in hepatic cells. Alcoholism-Clinical And Experimental Research, 2018, 42, 91A-91A.
  9. Huda N, Khambu B, Chen X and Yin XM. Autophagy regulates hepatocellular senescence. The FASEB Journal 2017;  31(1), 470.1-470.1
  10. Wang L, Zhou J, Chen X, Khambu B, Yin XM. Establishment of a cellular model to study ethanol-induced lipophagy. Hepatology 2016; 64, 615A-615A.
  11. Khambu B., Zhang, H. XY Chen, Y. Li and Yin XM.  Mechanism of Liver Injury caused by autophagy deficiency. The FASEB Journal; 29, 2015.
  12. Yang Z., Guo N., Khambu B., Zhang H., Liu P., Yin XM., Li M. Impaired lysosome function can induce autophagy via suppression of mTOR activity. The FASEB Journal 2014; 28 (S1), 738.1.
  13. Li, M, Khambu B, Zhang H, Vogt A..Liu P. and Yin XM. Suppression of Lysosome Function Induces Autophagy via a Feedback Downregulation of MTORC1 Activity. 2014 Keystone Meeting on Autophagy.
  14. Zhang H., Khambu B., Chen X., Yin XM. Modulation of Autophagy Affects the Hepatic Pathology in Alcoholic and Non-alcoholic Liver Diseases. The FASEB Journal 2013; 27, 1086.2.